Core A

Cutaneous Phenomics And Transcriptomics (CPAT)

***Important Notice***

Dear SBDRC Members,

Due to short-term staffing shortages, there will be temporary changes to services through the end of March for CPAT core requests. All services may be subject to increases in turnaround times, though we are working to minimalize the impact.  Two services will experience more limits:

    • LCM –  Trained users may still use the equipment; however, new user training, consultations, and slide preparation will be unavailable.
    • Special Stains – These will be handled on an ad hoc basis. Please inquire.  

We appreciate your patience during this period as we make every effort to continue providing quality services to the department and SBDRC community. 

Please contact SBDRC@pennmedicine.upenn.edu with any questions or concerns.

Regards,

   -Penn SBDRC

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The role of the Cutaneous Phenomics and Transcriptomics Core (CPAT) is to provide services and training necessary for the molecular characterization and histopathologic analysis of skin samples. Through both essential and innovative, state-of-the-art services CPAT Core’s mission it to provide investigators with key resources to determine the molecular and histologic features of skin and its diseases.

Expertise in skin tissue processing (FFPE and cryo) and microtomy are available, along with histochemical stains, immunoassays, RNAscope, LCM, and Barrier Testing services. For most services that are offered, protocols, consultation, training and equipment use is available. Additionally, the Core has access to an extensive archive of human skin disease and control samples, to provide investigators with FFPE clinical samples in the form of slides or ribbons/scrolls.

For convenience and efficiency, a comprehensive online form is used for submission of projects and requests, the details of which are automatically populated into a database, which is then used for tracking projects, core output, and billing.

Please note key changes effective July 1, 2024:

*Effective during normal operations, and subject to change 

 

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Skin tissue processing, microtomy, and staining (FFPE)
Archived human skin specimens (normal and diseased)
Cryo Sample Preparation and Immunostaining Services
Laser Capture Microdissection (LCM)
RNAscope in situ hybridization
Skin barrier testing (TEWL, pH, and corneometry)
Skin single cell suspension and library generation for scRNA-seq - COMING SOON
Intravital imaging (Two-photon microscopy and macrofluoresence) - COMING SOON